Library-free methylation sequencing with bisulfite padlock probes
نویسندگان
چکیده
منابع مشابه
RNA cytosine methylation analysis by bisulfite sequencing
Covalent modifications of nucleic acids play an important role in regulating their functions. Among these modifications, (cytosine-5) DNA methylation is best known for its role in the epigenetic regulation of gene expression. Post-transcriptional RNA modification is a characteristic feature of noncoding RNAs, and has been described for rRNAs, tRNAs and miRNAs. (Cytosine-5) RNA methylation has b...
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Genome-Wide Association Studies (GWAS) have identified genetic variants contributing to the risk of cardiovascular disease (CVD) at the chromosome 9p21 locus. The chromosome 9p21 is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery disease (CAD). F7 gene because of its role in activating the ...
متن کاملSMAP: a streamlined methylation analysis pipeline for bisulfite sequencing
BACKGROUND DNA methylation has important roles in the regulation of gene expression and cellular specification. Reduced representation bisulfite sequencing (RRBS) has prevailed in methylation studies due to its cost-effectiveness and single-base resolution. The rapid accumulation of RRBS data demands well designed analytical tools. FINDINGS To streamline the data processing of DNA methylation...
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DNA cytosine methylation is one of the most abundant epigenetic marks found in the plant nuclear genome. Bisulfite sequencing (BS-Seq) is the method of choice for profiling DNA cytosine methylation genome-wide at a single nucleotide resolution. The basis of this technique is that the unmethylated cytosine can be deaminated to uracil by sodium bisulfite, while the methylated cytosine is resistan...
متن کاملGenotyping RNA and DNA Using Padlock Probes
Antson, D.-O. 2001. Genotyping RNA and DNA using padlock probes. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1050. 40pp. Uppsala. ISBN 91-554-5057-1 Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable fo...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2012
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.1871